張亮生教授團隊還有若幹數據庫系統將面世發表，有望在此領域做出系統全面、前沿而實用的工作。另，張亮生教授課題組還發表了一篇數據庫領域的綜述文章，詳細解讀了各類型基因組數據庫的特點和異同。文章發表在Frontiers in Plant Science。上述工作主要貢獻者有陳飛博士及浙江農林大學王正加教授團隊。其中參與者中多名是本科生，張佳偉（大四，
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紅藻數據庫共同第一），李曉江（大三，紅藻數據庫共同第一），林梅桂（大四），郭欣悅（大四，推免到中科大）。相關成果和數據庫鏈接紅藻基因組和轉錄組數據庫 realDB：http://realdb.algaegenome.orgChen et al.錛 realDB: a genome and transcriptome resource for the red algae (phylum Rhodophyta). Database錛 Jan 1;2018. doi: 10.1093/database/bay072.文章網址：https://academic.oup.com/database/article/2018/1/bay072/5055577瑪咖基因組數據庫 MGH：http://maca.eplant.orgChen et al.錛 MGH: a genome hub for the medicinal plant maca (Lepidium meyenii). Database錛 Jan 1;2018. doi: 10.1093/database/bay113文章網址：https://academic.oup.com/database/article/2018/1/bay113/5136497被子植物數據庫http://angiosperms.org/Chen et al.錛 The Sequenced Angiosperm Genomes and Genome Databases. Front Plant Sci. doi: 10.3389/fpls.2018.00418文章網址：https://www.frontiersin.org/articles/10.3389/fpls.2018.00418/fullAuxinmediated Aux/IAAARFHB signaling cascade regulates secondary xylem development in PopulusFirst author: Changzheng Xu; Affiliations: Southwest University (西南大學): Chongqing錛 ChinaCorresponding author: Keming LuoWood development is strictly regulated by various phytohormones and auxin plays a central regulatory role in this process. However錛 it remains obscure how the auxin signaling is transducted in developing secondary xylem during wood formation in tree species. Here錛 we identified an Aux/INDOLE3ACETIC ACID 9 (IAA9)AUXIN RESPONSE FACTOR 5 (ARF5) module in Populus tomentosa as a key mediator of auxin signaling to control early developing xylem development. PtoIAA9錛 a canonical Aux/IAA gene錛 is predominantly expressed in vascular cambium and developing secondary xylem and induced by exogenous auxin. Overexpression of PtoIAA9m encoding a stabilized IAA9 protein significantly represses secondary xylem development in transgenic poplar. We further showed that PtoIAA9 interacts with PtoARF5 homologs via the C erminal III/IV domains. The truncated PtoARF5.1 protein without the III/IV domains rescued the PtoIAA9m esulting defective phenotypes. Expression analysis showed that the PtoIAA9PtoARF5 module regulated the expression of genes associated with secondary vascular development in PtoIAA9m and PtoARF5.1overexpressing plants. Furthermore錛 PtoARF5.1 could bind to the promoters of two Class III homeodomainleucine zipper (HDIP III) genes PtoHB7 and PtoHB8 to modulate secondary xylem formation. Taken together錛 our results suggest that the Aux/IAA9ARF5 module is required for auxin signaling to regulate wood formation via orchestrating the expression of HDIP III transcription factors in poplar.木材的發育受到多個激素的調控，而生長素在其中扮演著一個非常重要的作用。然而，林木的木材形成過程中生長素在發育的次生木質部中的信號轉導還不是很清楚。本文中，作者在毛白楊中鑑定到了一個IAA9-ARF5模塊作為生長素信號轉導的核心調控子，控制木質部的早期發育。PtoIAA9屬于典型的Aux/IAA基因，主要在維管形成層和發育中的木質部中表達，並且受到外源生長素處理的誘導。過表達PtoIAA9m基因編碼一個穩定的IAA9蛋白顯著抑制轉基因楊樹中的次生木質部的發育。進一步的試驗顯示PtoIAA9與PtoARF5同源蛋白通過C末端III/IV結構域發生互作。將III/IV結構域截掉了的PtoARF5.1蛋白能夠拯救PtoIAA9m所導致的缺陷表型。表達分析顯示PtoIAA9PtoARF5模塊在PtoIAA9m和PtoARF5.1過表達植株中調控參與次生維管發育相關基因的表達。此外，PtoARF5.1能夠結合到兩個HDIP III基因PtoHB7和PtoHB8的啟動子區，
